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Image Search Results
Journal: Reumatismo
Article Title: Anti-Ro and anti-La autoantibodies induce TNF-alpha production by human salivary gland cells: an in vitro study.
doi: 10.4081/reumatismo.2007.221
Figure Lengend Snippet: Figure 1 - Expression of the TNFR1 e TNFR2 receptors by the salivary gland cells. For RT-PCR analysis products of the expected size were vi- sualized through agarose gel electrophoresis. M, marker. RT PCR of GADPH was used as control (panel A). For confocal microscopy, cells we- re treated with biotinylated anti-human TNFR1 and biotinylated anti-human TNFR2. Streptavidin (FITC) was used for FITC secondary detection. Expression of TNFR1 (A), TNFR2 (B), untreated control cells (C), cells treated with streptavidin (FITC) (D). Scale bar = 10 µm.
Article Snippet: The cells were fixed with 2% (w/v) paraformaldehyde (SIGMA) in PBS for 10 min, and then treated with biotinylated anti-human TNFR1 and
Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker, Control, Confocal Microscopy
Journal: Frontiers in Veterinary Science
Article Title: Cytokine profile, differential somatic cell count, and oxidative status of Italian Mediterranean buffalo milk affected by the temperature–humidity index
doi: 10.3389/fvets.2024.1449017
Figure Lengend Snippet: Secretion of (a) IL-10, (b) INF-γ, (c) IL-1β, (d) TNF-α, (e) IL-12, (f) IL-4, (g) IL-12/IL-4 (Th1/Th2 ratio), and (h) IL-10/INF-γ ratio (Th2/Th1 ratio) in buffaloes’ milk whey under three THI classes (THI < 72, 72 ≤ THI < 76, and THI ≥ 76). The data are presented as mean ± SEM. Bars with different letters are significantly different at p < 0.05.
Article Snippet: Biotinylated anti-bovine IFN-γ antibody (Clone CC302, Bio-Rad Ltd., at a final concentration of 2 μg/mL) and
Techniques: